SH3RF1 antibody (Cy3)

Catalog No. ABIN1419023

The Rabbit Polyclonal anti-SH3RF1 antibody has been validated for WB, IF (p). It is suitable to detect SH3RF1 in samples from Human, Mouse, Rat.

Quick Overview for SH3RF1 antibody (Cy3) (ABIN1419023)

Target: SH3RF1 (SH3 Domain Containing Ring Finger 1 (SH3RF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3RF1 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SH3RF1 Antibody (Cy3)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SH3MD2/RNF142

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SH3RF1

Target: SH3RF1 (SH3 Domain Containing Ring Finger 1 (SH3RF1))

Alternative Name: RNF142

Background:

Synonyms: Plenty of SH3 domains, Plenty of SH3s, POSH, Ring finger protein 142, RNF 142, RNF142, SH3 domain containing ring finger 1, SH3 domain containing RING finger protein 1, SH3MD2, SH3 multiple domains 2, SH3RF1.

Background: SH3MD2 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3MD2 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane. This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. There are two named isoforms.

Gene ID: 57630