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PDZRN3 antibody (Cy3)

The Rabbit Polyclonal anti-PDZRN3 antibody has been validated for WB and IF (p). It is suitable to detect PDZRN3 in samples from Human, Mouse and Rat.
Catalog No. ABIN1419233

Quick Overview for PDZRN3 antibody (Cy3) (ABIN1419233)

Target

See all PDZRN3 Antibodies
PDZRN3 (PDZ Domain Containing Ring Finger 3 (PDZRN3))

Reactivity

  • 26
  • 18
  • 16
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Mouse, Rat

Host

  • 25
  • 1
Rabbit

Clonality

  • 26
Polyclonal

Conjugate

  • 9
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This PDZRN3 antibody is conjugated to Cy3

Application

  • 22
  • 12
  • 6
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
  • Cross-Reactivity

    Human, Mouse, Rat

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic peptide derived from human SEMCAP3/LNX3

    Isotype

    IgG
  • Application Notes

    IF(IHC-P) 1:50-200

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    PDZRN3 (PDZ Domain Containing Ring Finger 3 (PDZRN3))

    Alternative Name

    SEMCAP3

    Background

    Synonyms: Ligand of Numb protein X 3, likely ortholog of mouse semaF cytoplasmic domain associated protein 3, LNX3, PDZ domain containing ring finger 3, Protein SEMACAP3, SEMACAP3, Semaphorin cytoplasmic domain associated protein, PZRN3_HUMAN.

    Background: PDZRN3 contains a RING-finger motif in its N-terminal region, two PDZ domains in its central region and a consensus-binding motif for PDZ domains at its C-terminus. It was identified in silico as a homolog of the protein known as LNX1 or SEMCAP1, which possesses ubiquitin ligase activity and binds the membrane protein Semaphorin 4C. However, PDZRN3 itself has not previously been characterized. We have now evaluated the properties and functions of PDZRN3. The PDZRN3 gene was shown to be expressed in various human tissues including the heart, skeletal muscle and liver and its expression in mouse skeletal muscle was developmentally regulated. Both the differentiation of C2C12 mouse skeletal myoblasts into myotubes and injury-induced muscle regeneration in vivo were found to be accompanied by up-regulation of PDZRN3. The differentiation-associated increase in the expression of PDZRN3 in C2C12 cells follows that of myogenin and precedes that of myosin heavy chain. Depletion of PDZRN3 by RNA interference inhibited the formation of myotubes as well as the associated up-regulation of myosin heavy chain in C2C12 cells. Our data suggest that PDZRN3 plays an essential role in the differentiation of myoblasts into myotubes by acting either downstream or independently of myogenin.

    Gene ID

    23024

    Pathways

    Skeletal Muscle Fiber Development
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