PJA2 antibody (AA 420-470) (Cy3)

Catalog No. ABIN1419731

The Rabbit Polyclonal anti-PJA2 antibody has been validated for WB and IF (p). It is suitable to detect PJA2 in samples from Human, Mouse and Rat.

Quick Overview for PJA2 antibody (AA 420-470) (Cy3) (ABIN1419731)

Target: PJA2 (Praja Ring Finger 2 (PJA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PJA2 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PJA2 Antibody (Cy3)

Binding Specificity: AA 420-470

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PJA2/RNF131

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PJA2

Target: PJA2 (Praja Ring Finger 2 (PJA2))

Alternative Name: RNF131

Background:

Synonyms: E3 ubiquitin protein ligase Praja2, E3 ubiquitin-protein ligase Praja-2, KIAA0438, Neurodap 1, Neurodap1, PJA 2, PJA2, PJA2_HUMAN, Praja 2, Praja 2 RING H2 mot containing, praja ring finger 2, Praja ring finger 2 E3 ubiquitin protein ligase, Praja2, RING finger protein 131, RNF 131, RNF131.

Background: PJA2, also known as E3 ubiquitin-protein ligase praja-2, RNF131 (ring finger protein 131) or Neurodap1, is a 708 amino acid protein that contains one ring-type zinc finger and exists as two alternatively spliced isoforms. Significantly conserved in chimpanzee, dog, cow, mouse, rat, chicken and zebrafish, PJA2 shares 52 % identity with PJA1, which is involved in protein ubiquitination in brain and may play a role in X-linked mental retardation. Encoded by a gene that maps to human chromosome 5q21.3, PJA2 localizes to both endoplasmic reticulum and Golgi apparatus membranes. Participating in E2-dependent, E3 ubiquitin-protein ligase activity, PJA2 binds to a variety of E2s and interacts with ubiquitin-conjugating enzymes, such as UBE2D2, in vitro.

Gene ID: 9867

UniProt: O43164