PAPOL A+B+G antibody (HRP)

Catalog No. ABIN1420570

Product Details

Target: PAPOL A+B+G

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PAPOLA/PAPOLB/PAPOLG

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PAPOL A+B+G

Background:

Synonyms: PAPOL A/B/G, PAP alpha/beta/gamma, PapIII, MGC5378, PAP alpha, PAP beta, PAP gamma, PAP, PAP-alpha, PAP-beta, PAP-gamma, PAPOLA, PAPOLB, PAPOLG, PAPOA_HUMAN, PAPOB_HUMAN, PAPOG_HUMAN, Plap, polyA polymerase alpha, Polynucleotide adenylyltransferase alpha, polyA polymerase beta testis specic, polyA polymerase beta, polynucleotide adenylyltransferase beta, TPAP, Testis-specic polyA polymerase.

Background: Polyadenylation of the 3-prime ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction includes endoribonucleolytic cleavage of the pre-RNA at the poly(A) site that leads to synthesis of the poly(A) tail at the 3-prime end of the upstream cleavage product. The poly(A) polymerase (PAP) is required The adenosine addition reaction depends on poly(A) polymerase (PAP) activity. The testis express PAP-beta (TPAP) in the cytoplasm of spermatogenic cells. The adenosine addition function of PAP-beta plays a critical role in male germ cell production. PAP-beta-deficient transgenic mice display impaired expression of haploid-specific genes that are necessary for spermatogenesis. The intronless gene encoding human PAP-beta maps to chromosome 7p22.3.

Gene ID: 10914, 56903, 64895

UniProt: P51003, Q9NRJ5, Q9BWT3