B-Cell Linker antibody (pTyr189) (HRP)

Catalog No. ABIN1421968

This Rabbit Polyclonal antibody specifically detects B-Cell Linker in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for B-Cell Linker antibody (pTyr189) (HRP) (ABIN1421968)

Target: B-Cell Linker (BLNK)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B-Cell Linker antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-B-Cell Linker Antibody (HRP)

Binding Specificity: pTyr189

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human BLNK around the phosphorylation site of Tyr189 [EN(p-Y)IH]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background:

Synonyms: BLNK phospho Y491, p-BLNK phospho Y491, B cell adapter containing SH2 domain protein, B cell adapter containing Src homology 2 domain protein, B cell linker, B cell linker protein, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, B-cell linker protein, BASH, BASH, Bca, Bca, BLNK, BLNK s, BLNK_HUMAN, Cytoplasmic adapter protein, Ly 57, Ly-57, Ly57, Lymphocyte antigen 57, Lymphocyte antigen-57, Lyw 57, Lyw-57, Lyw57, MGC111051, SLP 65, SLP65, SLP-65, Src homology 2 domain containing leukocyte protein of 65 kDa.

Background: This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012].

Gene ID: 29760

Pathways: BCR Signaling