PDE4A antibody (HRP)

Catalog No. ABIN1422148

This Rabbit Polyclonal antibody specifically detects PDE4A in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PDE4A antibody (HRP) (ABIN1422148)

Target: PDE4A (phosphodiesterase 4A, CAMP-Specific (PDE4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE4A antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDE4A Antibody (HRP)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PDE4A8

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDE4A

Target: PDE4A (phosphodiesterase 4A, CAMP-Specific (PDE4A))

Background:

Synonyms: 2EL, DPDE2, PDE4, PDE46, PDE4A, Phosphodiesterase 4A, Phosphodiesterse Type 4A8, PDE4A_HUMAN.

Background: The PDE4A8 is a testis germ cell-specific PDE4A variant. There are two PDE4A variants identified in rat testis (PDE4A8 and an un-characterized 76 kDa PDE4A protein). Spermatogenesis is accompanied by expression of a varied repertoire of PDE4 enzymes at different stages of cell differentiation that presumably perform specialized functions. Confocal microscopic examination revealed the presence of PDE4A8 in primary and secondary spermatids but not in mature spermatozoa. PDE4A8 inhibition results in increased apoptosis of the germ cells accompanied with reduction in the number of mature spermatozoa.

Gene ID: 5141

Pathways: cAMP Metabolic Process