HCN3 antibody (HRP)

Catalog No. ABIN1422934

This Rabbit Polyclonal antibody specifically detects HCN3 in WB and IHC (p). It exhibits reactivity toward Mouse, Rat and Human.

Quick Overview for HCN3 antibody (HRP) (ABIN1422934)

Target: HCN3 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 3 (HCN3))

Reactivity: Mouse, Rat, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCN3 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HCN3 Antibody (HRP)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HCN3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HCN3

Target: HCN3 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 3 (HCN3))

Alternative Name: HCN3

Background:

Synonyms: Hcn3, HCN3_HUMAN, hyperpolarization activated cyclic nucleotide-gated potassium channel 3, KIAA1535, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3.

Background: Hyperpolarization-activated, cyclic nucleotide-binding channels (HCN) are voltage-gated cation channels that are activated by direct binding of intracellular cyclic nucleotides. The HCN family consists of four members (HCN1-4), each with a core transmembrane segment domain and a C-terminal 120 amino-acid cyclic nucleotide-binding domain motif. HCN channels are expressed in the brain, heart, thalamus and testis. The pacemaker properties of HCN channels contribute to spontaneous rhythmic activity in the brain and heart. HCN3 contains a segment characterized by a series of positively charged amino acids at every third position. This region designated S4 is likely to be the voltage sensor of the protein. In the brain, HCN3 and HCN4 exhibit subcortical distribution mainly concentrated in the hypothalamus and thalamus, respectively.

Gene ID: 57657