MARCH3 antibody (PE-Cy7)

Catalog No. ABIN1435531

This Rabbit Polyclonal antibody specifically detects MARCH3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Chicken and Pig.

Quick Overview for MARCH3 antibody (PE-Cy7) (ABIN1435531)

Target: MARCH3 (Membrane-Associated Ring Finger (C3HC4) 3, E3 Ubiquitin Protein Ligase (MARCH3))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH3 antibody is conjugated to PE-Cy7

Application: Western Blotting (WB)

Product Details anti-MARCH3 Antibody (PE-Cy7)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MARCH3

Isotype: IgG

Application Details

Application Notes: FCM(1:100-500)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MARCH3

Target: MARCH3 (Membrane-Associated Ring Finger (C3HC4) 3, E3 Ubiquitin Protein Ligase (MARCH3))

Alternative Name: MARCH3

Background:

Synonyms: E3 ubiquitin-protein ligase MARCH3, FLJ20096, MARCH-III, March3, MARH3_HUMAN, Membrane associated ring finger C3HC4 3, Membrane-associated RING finger protein 3, Membrane-associated RING-CH protein III, RING finger protein 173, RNF173.

Background: Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). MARCH3 (membrane-associated ring finger (C3HC4) 3), also known as RNF173, is a 253 amino acid multi-pass membrane protein that localizes to cytoplasmic vesicles and early endosomes and contains one RING-CH-type zinc finger. Involved in the pathway of protein modification, MARCH3 functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and is thought to be involved in endosomal trafficking events.

Molecular Weight: 24kDa

Gene ID: 115123