RAB27A antibody (C-Term)

Catalog No. ABIN1440000

Cited in 3+ publications. The Goat Polyclonal anti-RAB27A antibody (ABIN1440000) specifically detects RAB27A in WB, IF, IHC (p) and IHC (fro). The antibody is reactive with Human, Mouse, Rat, Cow, Goat, Horse, Sheep, Dog, Guinea Pig, Rabbit, Monkey, Chicken, Pig, Cat, Donkey, Hamster and Avian samples.

Quick Overview for RAB27A antibody (C-Term) (ABIN1440000)

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Reactivity: Human, Mouse, Rat, Cow, Goat, Horse, Sheep, Dog, Guinea Pig, Rabbit, Monkey, Chicken, Pig, Cat, Donkey, Hamster, Avian

Host: Goat

Clonality: Polyclonal

Conjugate: This RAB27A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RAB27A Antibody

Binding Specificity: C-Term

Purpose: Anti-Rab27a

Sequence: MHAYCENPDI VLCGNKSDLE DQRAVKEEEA RELAEKYGIP YFETSAANGT NISQAIEMLL DLIMKRMERC VDKSWIPEGV VRSNGHTSTD QLSEEKEKGL CGC

Specificity: Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a. This antibody also recognizes Rab27b protein.

Characteristics: RAB27A, member RAS oncogene family

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:250-1:2,000, IF:1:50-1:200, IHC-P:1:50-1:400, IHC-F:1:50-1:400

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-RAB27A antibody (ABIN1440000)

Alzahofi, Welz, Robinson, Page, Briggs, Stainthorp, Reekes, Elbe, Straub, Kallemeijn, Tate, Goff, Sviderskaya, Cantero, Montoliu, Nedelec, Miles, Bailly, Kerkhoff, Hume: "Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins." in: Nature communications, Vol. 11, Issue 1, pp. 3495, (2020) (PubMed).

Pelkonen, Reinisalo, Morin-Picardat, Kidron, Urtti: "Isolation of Intact and Functional Melanosomes from the Retinal Pigment Epithelium." in: PLoS ONE, Vol. 11, Issue 8, pp. e0160352, (2017) (PubMed).

Reichhart, Markowski, Ishiyama, Wagner, Crespo-Garcia, Schorb, Ramalho, Milenkovic, Föckler, Seabra, Strauß: "Rab27a GTPase modulates L-type Ca2+ channel function via interaction with the II-III linker of CaV1.3 subunit." in: Cellular signalling, Vol. 27, Issue 11, pp. 2231-40, (2016) (PubMed).

Target Details for RAB27A

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Alternative Name: Rab27a

Background:

Goat polyclonal antibody to mouse Rab27a. Rab27a belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in lysosome-related vesicle transport and small GTPase mediated signal transduction.

GS2, GTP-binding protein Ram, rab-27, ras-related protein Rab-27A, RAB27, RAM antibody.

Pathways: Peptide Hormone Metabolism