Calnexin antibody (C-Term)

Catalog No. ABIN1440006

Product Details anti-Calnexin Antibody

Target: Calnexin (CANX)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Pig, Monkey, Hamster, Sheep, Chicken, Avian, Horse, Goat, Cat, Donkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.

Characteristics: Calnexin

Purification: epitope affinity purified

Immunogen: Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: Polyclonal antibody supplied as a 200 µL (2 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-Calnexin antibody (ABIN1440006)

Rodrigues, Matafome, Sereno, Almeida, Castelhano, Gamas, Neves, Gonçalves, Carvalho, Arslanagic, Wilcken, Fonseca, Simões, Conde, Castelo-Branco, Seiça: "Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance." in: Scientific reports, Vol. 7, Issue 1, pp. 1698, (2018) (PubMed).

Thieleke-Matos, Lopes da Silva, Cabrita-Santos, Portal, Rodrigues, Zuzarte-Luis, Ramalho, Futter, Mota, Barral, Seabra: "Host cell autophagy contributes to Plasmodium liver development." in: Cellular microbiology, Vol. 18, Issue 3, pp. 437-50, (2016) (PubMed).

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: CANX (CANX Products)

Background: CANX is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, ER-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
Synonyms: Calnexin, CALX, CNX, FLJ26570, histocompatibility complex class I antigen binding protein p88, IP90, major histocompatibility complex class I antigen-binding protein p88, MS952, P90 antibody.
Ensembl identifier: ENSG00000127022

Pathways: MAPK Signaling, Thyroid Hormone Synthesis