GAPDH antibody (C-Term)

Catalog No. ABIN1440016

This anti-GAPDH antibody is a Goat Polyclonal antibody detecting GAPDH in WB, IF, IHC (p) and IHC (fro). Suitable for Human, Mouse, Rat, Rabbit, Pig, Chicken, Dog, Monkey, Sheep, Cow, Hamster, Cat, Horse, Guinea Pig, Goat, Fish, Avian and Donkey. This Primary Antibody has been cited in 8+ publications.

Quick Overview for GAPDH antibody (C-Term) (ABIN1440016)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Dog, Monkey, Sheep, Cow, Hamster, Cat, Horse, Guinea Pig, Goat, Fish, Avian, Donkey

Host: Goat

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GAPDH Antibody

Binding Specificity: C-Term

Specificity: Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.

Characteristics: Glyceraldehyde-3-phosphate dehydrogenase

Purification: epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: Polyclonal antibody supplied as a 200 µL (2 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-GAPDH antibody (ABIN1440016)

Ferreira, Rosa Soares, Ramalho, Ribeiro-Rodrigues, Máximo, Zuzarte, Girão, Pereira: "Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis." in: PLoS ONE, Vol. 14, Issue 10, pp. e0223790, (2020) (PubMed).

Sanzà, Evans, Briggs, Cantero, Montoliu, Patel, Sviderskaya, Itzen, Figueiredo, Seabra, Hume: "Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a." in: Journal of cell science, Vol. 132, Issue 9, (2020) (PubMed).

Aires, Boia, Rodrigues-Neves, Madeira, Marques, Ambrósio, Santiago: "Blockade of microglial adenosine A2A receptor suppresses elevated pressure-induced inflammation, oxidative stress, and cell death in retinal cells." in: Glia, Vol. 67, Issue 5, pp. 896-914, (2019) (PubMed).

Robinson, Evans, Briggs, Ramalho, Hume: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." in: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).

Ramalho, Toscano, Pereira, Girão, Gonçalves, Marques: "Hyperglycemia-induced degradation of HIF-1α contributes to impaired response of cardiomyocytes to hypoxia." in: Revista portuguesa de cardiologia : orgão oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology, Vol. 36, Issue 5, pp. 367-373, (2018) (PubMed).

Ferreira, Soares, Ramalho, Pereira, Girao: "K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy." in: Scientific reports, Vol. 5, pp. 10210, (2016) (PubMed).

Casalou, Seixas, Portelinha, Pintado, Barros, Ramalho, Lopes, Barral: "Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration." in: Journal of cell science, Vol. 127, Issue Pt 12, pp. 2709-22, (2015) (PubMed).

Ribeiro-Rodrigues, Catarino, Marques, Ferreira, Martins-Marques, Pereira, Girão: "AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 11, pp. 4629-41, (2015) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Synonyms: glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.
Ensembl identifier: ENSG00000111640