IL-18 antibody (AA 37-194)

Catalog No. ABIN1449292

This Mouse Monoclonal antibody specifically detects IL-18 in WB. It exhibits reactivity toward Mouse and Rat.

Quick Overview for IL-18 antibody (AA 37-194) (ABIN1449292)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 21A12

Product Details anti-IL-18 Antibody

Binding Specificity: AA 37-194

Specificity: This antibody reacts with IL-18.

Cross-Reactivity (Details): Species reactivity (tested):Rat, weakly with Mouse. Does not react with Human (recombinant).

Purification: Protein A agarose

Immunogen: Mature rat IL-18 fusion protein corresponding to 37-194 aa

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, store (in aliqouts) at -20 °C.

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: Interleukin-18 / IL18

Background: Interleukin 18 (IL-18) is an 18- kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24- kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-γ also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.Synonyms: IGIF, IL-1 gamma, IL-18, IL1 gamma, IL1F4, Iboctadekin, Interferon-gamma-inducing factor, Interleukin-1 gamma

Gene ID: 29197

NCBI Accession: NP_062038

UniProt: P97636

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome