LC3C antibody (N-Term)

Catalog No. ABIN1449628

This Rabbit Polyclonal antibody specifically detects LC3C in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for LC3C antibody (N-Term) (ABIN1449628)

Target: LC3C (MAP1LC3C) (Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-LC3C Antibody

Binding Specificity: AA 1-30, N-Term

Purification: Saturated Ammonium Sulfate precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human LC3

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LC3C

Target: LC3C (MAP1LC3C) (Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C))

Alternative Name: MAP1LC3C

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3c is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.Synonyms: Autophagy-related protein LC3 C, Autophagy-related ubiquitin-like modifier LC3 C, MAP1 light chain 3-like protein 3, MAP1A/1B light chain 3 C, MAP1A/MAP1B LC3 C, Microtubule-associated protein 1 LC3 gamma, Microtubule-associated proteins 1A/1B light chain 3C

Gene ID: 440738

NCBI Accession: NP_001004343

Pathways: Autophagy