SPARC antibody

Catalog No. ABIN1450173

This anti-SPARC antibody is a Mouse Monoclonal antibody detecting SPARC in WB, FACS, IF, IHC (p), IHC (fro), EIA and RIA. Suitable for Human, Rat and Cow.

Quick Overview for SPARC antibody (ABIN1450173)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Clone: N50

Product Details anti-SPARC Antibody

Specificity: Recognizes Human and Bovine bone Osteonectin, Human platelet Osteonectin. SPARC produced by various cells grown in culture. Cross reacts with Rat Osteonectin.Aff. Const.:5 x 10e-9 M

Purification: Ammonium Sulfate Precipitation, Ion Exchange Chromatography and Gel Filtration.

Immunogen: Crude bone extract containing native Osteonectin.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 10.8 mg/mL (OD280 nm, E1% = 14)

Buffer: 50 % Glycerol/H2O (vol/vol), None

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC / Osteonectin

Background: SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules. Tumor growth is enhanced in mice lacking SPARC due to changes in the ECM that create a more permissive environment for tumor progression.Synonyms: BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine

Gene ID: 6678

NCBI Accession: NP_003109

UniProt: P09486

Pathways: Autophagy