NOS1 antibody (N-Term)

Catalog No. ABIN1495363

This Rabbit Polyclonal antibody specifically detects NOS1 in WB, IHC, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for NOS1 antibody (N-Term) (ABIN1495363)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NOS1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Recognizes the ~160kD nNOS protein and does not exhibit any cross-reactivity with the related eNOS or iNOS proteins.

Purification: Immunoaffinity purified

Immunogen: A recombinant protein consisting of 195 amino acids from the N-terminal of rat nNOS protein.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1.0 μg/mL. Western Blot: 1 μg/mL. Antibody reacts with a ~160 kD band on Western blots of rat and mouse brain tissue lysates (20 μg). Immunohistochemistry (paraffin): 5-10 μg/mL. Immunohistochemistry (frozen): 1-2 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: NOS1 / nNOS

Background: Name/Gene ID: NOS1

Synonyms: NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i

Gene ID: 4842

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction