GNB3 antibody (AA 309-321)

Catalog No. ABIN1496050

This Goat Polyclonal antibody specifically detects GNB3 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Chicken, Cow, Dog, Rabbit, Xenopus laevis, Monkey, Bat, Horse and Pig.

Quick Overview for GNB3 antibody (AA 309-321) (ABIN1496050)

Target: GNB3 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 3 (GNB3))

Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Rabbit, Xenopus laevis, Monkey, Bat, Horse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This GNB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GNB3 Antibody

Binding Specificity: AA 309-321

Brand: IHC-plus™

Specificity: Human GNB3. This antibody is expected to recognize GNB3 & GNB4 (GeneID: 2784, 59345, ProteinID: NP_002066.1, NP_067642.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Turkey, Chicken, Xenopus (100%) Hamster, Elephant, Opossum, Lizard, Catfish, Salmon, Stickleback, Medaka, Pufferfish, Zebrafish, Drosophila, Nematode (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide SGHDNRVSCLGVT from an internal region of human GNB3 (NP_002066.1, NP_067642.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Turkey, Chicken, Xenopus (100%), Hamster, Elephant, Opossum, Lizard, Catfish, Salmon, Stickleback, Medaka, Pufferfish, Zebrafish, Drosophila, Nematode (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:16000), WB (0.1 - 0.3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 35kD band observed in lysates of cell line HepG2 (calculated MW of 37.2kD according to NP_002066.1. Recommended concentration: 0.1-0.3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GNB3

Target: GNB3 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 3 (GNB3))

Alternative Name: GNB3

Background: Name/Gene ID: GNB3

Synonyms: GNB3, G protein, beta-3 subunit, Transducin beta chain 3

Gene ID: 2784

NCBI Accession: NP_002066, NP_067642

UniProt: P16520

Pathways: Peptide Hormone Metabolism, Myometrial Relaxation and Contraction