APOBEC3F antibody (AA 34-263)

Catalog No. ABIN1496666

The Rabbit Polyclonal anti-APOBEC3F antibody is suitable to detect APOBEC3F in samples from Human. It has been validated for WB, IHC, IHC (p), IF and ICC.

Quick Overview for APOBEC3F antibody (AA 34-263) (ABIN1496666)

Target: APOBEC3F (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3F (APOBEC3F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOBEC3F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-APOBEC3F Antibody

Binding Specificity: AA 34-263

Specificity: Human APOBEC3F

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 34 and 263 (Q8IUX4) of APOBEC3F. Percent identity by BLAST analysis:

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:1000), IF (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for APOBEC3F

Target: APOBEC3F (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3F (APOBEC3F))

Alternative Name: APOBEC3F / ARP8

Background: Name/Gene ID: APOBEC3F

Synonyms: APOBEC3F, BK150C2.4.MRNA, ARP8, Induced upon T-cell activation, KA6

Gene ID: 200316

UniProt: Q8IUX4