ARHGEF25/GEFT antibody (AA 226-446)

Catalog No. ABIN1496711

The Rabbit Polyclonal anti-ARHGEF25/GEFT antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ARHGEF25/GEFT in samples from Human.

Quick Overview for ARHGEF25/GEFT antibody (AA 226-446) (ABIN1496711)

Target: ARHGEF25/GEFT (ARHGEF25) (Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF25/GEFT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARHGEF25/GEFT Antibody

Binding Specificity: AA 226-446

Specificity: Human ARHGEF25 / GEFT

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse (86%).

Purification: Immunoaffinity purified

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 226 and 446 of GEFT. Percent identity by BLAST analysis: Human (100%), Mouse (86%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for ARHGEF25/GEFT

Target: ARHGEF25/GEFT (ARHGEF25) (Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25))

Alternative Name: ARHGEF25 / GEFT

Background: Name/Gene ID: ARHGEF25

Synonyms: ARHGEF25, GEFT, RhoA/RAC/CDC42 exchange factor, p63RhoGEF, RAC/CDC42 exchange factor

Gene ID: 115557

UniProt: Q86VW2