CHAD antibody (AA 76-307)

Catalog No. ABIN1497487

This Rabbit Polyclonal antibody specifically detects CHAD in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CHAD antibody (AA 76-307) (ABIN1497487)

Target: CHAD (Chondroadherin (CHAD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHAD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHAD Antibody

Binding Specificity: AA 76-307

Specificity: Human CHAD

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 76 and 307 of CHAD (SwissProt O15335). Percent identity by BLAST analysis:

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000[suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for CHAD

Target: CHAD (Chondroadherin (CHAD))

Alternative Name: Chondroadherin / CHAD

Background: Name/Gene ID: CHAD

Synonyms: CHAD, Cartilage leucine-rich protein, Chondroadherin, SLRR4A, Chondroadherin proteoglycan

Gene ID: 1101

UniProt: O15335