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DARS antibody (AA 304-501)

This anti-DARS antibody is a Rabbit Polyclonal antibody detecting DARS in WB, IHC and IHC (p). Suitable for Human.
Catalog No. ABIN1497768

Quick Overview for DARS antibody (AA 304-501) (ABIN1497768)

Target

See all DARS Antibodies
DARS (Aspartyl-tRNA Synthetase (DARS))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This DARS antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

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    AA 304-501

    Specificity

    Human DARS

    Predicted Reactivity

    Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Bovine (97%) Chicken, Xenopus (94%) Zebrafish (92%).

    Purification

    Immunoaffinity purified

    Immunogen

    Recombinant fragment corresponding to a region within amino acids 304 and 501 of DARS (SwissProt P14868). Percent identity by BLAST analysis: Human (100%), Mouse, Rat, Bovine (97%), Chicken, Xenopus (94%), Zebrafish (92%).

    Type of Immunogen: Recombinant protein

    Isotype

    IgG
  • Application Notes

    Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

    Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    0.1 M Tris-glycine,  pH 7, 20 % Glycerol, 0.01 % Thimerosal

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid freeze-thaw cycles.

    Storage

    -20 °C

    Storage Comment

    Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
  • Target

    DARS (Aspartyl-tRNA Synthetase (DARS))

    Alternative Name

    DARS

    Background

    Name/Gene ID: DARS

    Synonyms: DARS, Aspartyl-tRNA synthetase, AspRS

    Gene ID

    1615

    UniProt

    P14868
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