Keratin 72 antibody (AA 217-434)

Catalog No. ABIN1499071

This Rabbit Polyclonal antibody specifically detects Keratin 72 in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Keratin 72 antibody (AA 217-434) (ABIN1499071)

Target: Keratin 72 (KRT72)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 72 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Keratin 72 Antibody

Binding Specificity: AA 217-434

Specificity: Human KRT6 / Cytokeratin 6

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine (89%).

Purification: Immunoaffinity purified

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 217 and 434 of Cytokeratin 72. Percent identity by BLAST analysis: Human (100%), Bovine (89%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for Keratin 72

Target: Keratin 72 (KRT72)

Alternative Name: KRT72 / Keratin 72 / K6irs

Background: Name/Gene ID: KRT72

Synonyms: KRT72, CK-72, K6irs, K72, Keratin protein K6irs, K6IRS2, KRT6IRS2, KB35, Keratin 72, Type-II keratin Kb35, Cytokeratin-72, Keratin-72, KRT6

Gene ID: 140807