CCL8 antibody (AA 24-99)

Catalog No. ABIN1499347

This Mouse Monoclonal antibody specifically detects CCL8 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CCL8 antibody (AA 24-99) (ABIN1499347)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CCL8 Antibody

Binding Specificity: AA 24-99

Brand: IHC-plus™

Specificity: Human MCP-2

Purification: Protein A purified

Immunogen: E.coli derived recombinant Human MCP-2 cprresponding to amino acids 24-99 of P80075.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1 - 2 μg/mL), IHC, IHC-P (10 μg/mL), WB (0.25 - 0.5 μg/mL)

Usage: Immunohistochemistry: LS-B8198 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 μg/mL is recommended. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1-2 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-2 when used with biotinylated antigen affinity purified anti-human MCP-2 (LS-C104731) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: CCL8 / MCP-2

Background: Name/Gene ID: CCL8
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL8, C-C motif chemokine 8, HC14, MCP2, Monocyte chemotactic protein 2, Scya10, SCYA8, Small-inducible cytokine A8, MCP-2, Chemokine (C-C motif) ligand 8

Gene ID: 6355

UniProt: P80075