PPIF antibody (AA 21-207)

Catalog No. ABIN1500361

This Rabbit Polyclonal antibody specifically detects PPIF in WB, IHC, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PPIF antibody (AA 21-207) (ABIN1500361)

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPIF Antibody

Binding Specificity: AA 21-207

Specificity: Human PPIF / Cyclophilin F

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rat (98%) Mouse (97%) Chicken (95%).

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 21 and 207 of PPIF (SwissProt P30405). Percent identity by BLAST analysis: Human (100%), Rat (98%), Mouse (97%), Chicken (95%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:1000), IF (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for PPIF

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Alternative Name: PPIF / Cyclophilin F

Background: Name/Gene ID: PPIF

Synonyms: PPIF, Cyclophilin 3, CYP3, Cyp-D, HCyP3, Peptidylprolyl isomerase F, Rotamase F, PPIase F, Cyclophilin D, Cyclophilin F

Gene ID: 10105

UniProt: P30405

Pathways: Proton Transport, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity