alpha Adducin antibody (pSer726)

Catalog No. ABIN1531171

The Rabbit Polyclonal anti-alpha Adducin antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect alpha Adducin in samples from Human, Mouse and Rat.

Quick Overview for alpha Adducin antibody (pSer726) (ABIN1531171)

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Adducin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-alpha Adducin Antibody

Binding Specificity: AA 688-737, pSer726

Specificity:

ADD1 (Phospho-Ser726) Antibody detects endogenous levels of ADD1 only when phosphorylated at Ser726.

PhosphorylationH:S726/S713 M:S724/S712 R:S724/S712

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

Comment:

Unigene-Number: Hs.183706, Hs.701490, Hs.188528 (NCBI Gene Symbol: ADD1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for alpha Adducin

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Alternative Name: ADD1

Background:

Synonyms: Alpha-adducin, Erythrocyte adducin subunit alpha , ADD1 , ADDA

NCBI Gene Symbol: ADD1

Molecular Weight: 80 kDa

Gene ID: 118, 119

OMIM: 102680

UniProt: P35611, P35612

Pathways: Negative Regulation of Hormone Secretion, Regulation of Actin Filament Polymerization, Regulation of Lipid Metabolism by PPARalpha, ER-Nucleus Signaling