AMPK alpha antibody (pThr183)

Catalog No. ABIN1531172

This anti-AMPK alpha antibody is a Rabbit Polyclonal antibody detecting AMPK alpha in WB, IHC and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for AMPK alpha antibody (pThr183) (ABIN1531172)

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMPK alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-AMPK alpha Antibody

Binding Specificity: AA 140-189, pThr183

Specificity:

AMPK alpha (Phospho-Thr172) Antibody detects endogenous levels of AMPK alpha only when phosphorylated at Thr172.

PhosphorylationH:T183/172 M:T183/172 R:T183/172

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

Comment:

Unigene-Number: Hs.43322, Hs.437039 (NCBI Gene Symbol: AAPK1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for AMPK alpha

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Alternative Name: AMPK alpha

Background:

Synonyms: 5'-AMP-activated protein kinase, catalytic alpha-1 chain, AAPK1, AMPK alpha-1 chain, AMPK-alpha1, HMG-CoA redustase kinase, PRKAA1, acetyl-CoA carboxylase kinase, hormone-sensitive lipase kinase, kinase AMPK-alpha1, 5'-AMP-activated protein kinase catalyt

NCBI Gene Symbol: AAPK1

Molecular Weight: 62 kDa

Gene ID: 5562, 5563

OMIM: 602739

UniProt: Q13131, P54646

Pathways: Warburg Effect