AMPK alpha antibody (pThr183)

Catalog No. ABIN1531172

The Rabbit Polyclonal anti-AMPK alpha antibody (ABIN1531172) specifically detects AMPK alpha in WB, IHC and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for AMPK alpha antibody (pThr183) (ABIN1531172)

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMPK alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-AMPK alpha Antibody

Binding Specificity: AA 140-189, pThr183

Specificity:

AMPK alpha (Phospho-Thr172) Antibody detects endogenous levels of AMPK alpha only when phosphorylated at Thr172.

PhosphorylationH:T183/172 M:T183/172 R:T183/172

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

Comment:

Unigene-Number: Hs.43322, Hs.437039 (NCBI Gene Symbol: AAPK1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for AMPK alpha

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Alternative Name: AMPK alpha

Background:

Synonyms: 5'-AMP-activated protein kinase, catalytic alpha-1 chain, AAPK1, AMPK alpha-1 chain, AMPK-alpha1, HMG-CoA redustase kinase, PRKAA1, acetyl-CoA carboxylase kinase, hormone-sensitive lipase kinase, kinase AMPK-alpha1, 5'-AMP-activated protein kinase catalyt

NCBI Gene Symbol: AAPK1

Molecular Weight: 62 kDa

Gene ID: 5562, 5563

OMIM: 602739

UniProt: Q13131, P54646

Pathways: Warburg Effect