LIPE antibody (pSer855)

Catalog No. ABIN1531230

This Rabbit Polyclonal antibody specifically detects LIPE in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LIPE antibody (pSer855) (ABIN1531230)

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIPE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-LIPE Antibody

Binding Specificity: AA 520-569, pSer855

Specificity:

HSL (Phospho-Ser855/554) Antibody detects endogenous levels of HSL only when phosphorylated at Ser855/554.

PhosphorylationH:S855 M:S559 R:S865

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HSL around the phosphorylation site of Ser855/554.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.656980 (NCBI Gene Symbol: LIPE)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for LIPE

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Alternative Name: HSL

Background:

Synonyms: Hormone sensitive lipase, LIPE, LIPS

NCBI Gene Symbol: LIPE

Molecular Weight: 116 kDa

Gene ID: 3991

OMIM: 151750

UniProt: Q05469

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism