IKK-alpha /IKK-beta antibody (pSer180)

Catalog No. ABIN1531284

This Rabbit Polyclonal antibody specifically detects IKK-alpha /IKK-beta in WB, IHC and ELISA. It exhibits reactivity toward Mouse, Human and Ratand has been mentioned in 1 publication.

Quick Overview for IKK-alpha /IKK-beta antibody (pSer180) (ABIN1531284)

Target: IKK-alpha /IKK-beta

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK-alpha /IKK-beta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IKK-alpha /IKK-beta Antibody

Binding Specificity: AA 151-200, pSer180

Specificity:

IKK-alpha/beta (Phospho-Ser180/181) Antibody detects endogenous levels of IKK-alpha/beta only when phosphorylated at Ser180/181.

PhosphorylationH:S180/S181 M:S180/S181 R:S180/S181

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta around the phosphorylation site of Ser180/181.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.198998, Hs.656458 (NCBI Gene Symbol: CHUK)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-IKK-alpha /IKK-beta antibody (ABIN1531284)

Hsieh, Hsiao, Hsu, Wang, Sheu: "PMC, a potent hydrophilic ?-tocopherol derivative, inhibits NF-?B activation via PP2A but not I?B?-dependent signals in vascular smooth muscle cells." in: Journal of cellular and molecular medicine, Vol. 18, Issue 7, pp. 1278-89, (2014) (PubMed).

Target Details for IKK-alpha /IKK-beta

Target: IKK-alpha /IKK-beta

Alternative Name: IKK-alpha/beta

Background:

Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IkappaB kinase, IkBKA, IKK-A, IKK-alpha, IKK1, IKKA, Inhibitor of nuclear factor kappa-B kinase alpha subunit, kinase IKK-alpha, NFKBIKA, Nuclear factor NFkapp

NCBI Gene Symbol: CHUK

Molecular Weight: 84 kDa

Gene ID: 1147, 3551

OMIM: 600664

UniProt: O15111, O14920