CBL antibody (pTyr700)

Catalog No. ABIN1531307

The Rabbit Polyclonal anti-CBL antibody (ABIN1531307) specifically detects CBL in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CBL antibody (pTyr700) (ABIN1531307)

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CBL Antibody

Binding Specificity: AA 666-715, pTyr700

Specificity:

CBL (Phospho-Tyr700) Antibody detects endogenous levels of CBL only when phosphorylated at Tyr700.

PhosphorylationH:Y700 M:Y698 R:Y806

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CBL around the phosphorylation site of Tyr700.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.504096 (NCBI Gene Symbol: CBL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for CBL

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Alternative Name: CBL

Background:

Synonyms: Cas-Br-M (murine) ecotropic retroviral transforming, CBL E3 ubiquitin protein ligase, CBL2, Proto-oncogene c-CBL, Signal transduction protein CBL

NCBI Gene Symbol: CBL

Molecular Weight: 99 kDa

Gene ID: 867

OMIM: 165360

UniProt: P22681

Pathways: TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals