CD247 antibody (pTyr142)

Catalog No. ABIN1531309

The Rabbit Polyclonal anti-CD247 antibody is suitable to detect CD247 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for CD247 antibody (pTyr142) (ABIN1531309)

Target: CD247 (CD247 Molecule (CD247))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD247 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD247 Antibody

Binding Specificity: AA 111-160, pTyr142

Specificity:

CD3 zeta (Phospho-Tyr142) Antibody detects endogenous levels of CD3 zeta only when phosphorylated at Tyr142.

PhosphorylationH:Y142 M:Y142 R:Y142

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CD3 zeta around the phosphorylation site of Tyr142.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:1000

Comment:

Unigene-Number: Hs.156445 (NCBI Gene Symbol: CD247)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for CD247

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD3 zeta

Background:

Synonyms: CD3-zeta, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain precursor, T3Z, TCRZ

NCBI Gene Symbol: CD247

Molecular Weight: 18 kDa

Gene ID: 919

OMIM: 186780

UniProt: P20963

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway