PDE4D antibody (pSer190)

Catalog No. ABIN1531369

The Rabbit Polyclonal anti-PDE4D antibody has been validated for WB, ELISA and IHC. It is suitable to detect PDE4D in samples from Human, Mouse and Rat.

Quick Overview for PDE4D antibody (pSer190) (ABIN1531369)

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE4D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PDE4D Antibody

Binding Specificity: AA 156-205, pSer190

Specificity:

PDE4D (Phospho-Ser190/53) Antibody detects endogenous levels of PDE4D only when phosphorylated at Ser190/53.

PhosphorylationH:S190 M:S129 R:S185

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PDE4D around the phosphorylation site of Ser190/53.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.117545 (NCBI Gene Symbol: PDE4D)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PDE4D

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Alternative Name: PDE4D

Background:

Synonyms: DPDE3, PDE43, PDE4D4, cAMP-specific 3',5'-cyclic phosphodiesterase 4D

NCBI Gene Symbol: PDE4D

Molecular Weight: 91 kDa

Gene ID: 5144

OMIM: 600129

UniProt: Q08499

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling