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EIF4B antibody (pSer422)

This anti-EIF4B antibody is a Rabbit Polyclonal antibody detecting EIF4B in WB, ELISA, IHC and IF. Suitable for Human and Mouse.
Catalog No. ABIN1531431

Quick Overview for EIF4B antibody (pSer422) (ABIN1531431)

Target

See all EIF4B Antibodies
EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity

  • 98
  • 50
  • 35
  • 6
  • 6
  • 4
  • 4
  • 3
  • 2
  • 1
Human, Mouse

Host

  • 84
  • 12
  • 2
Rabbit

Clonality

  • 82
  • 16
Polyclonal

Conjugate

  • 57
  • 6
  • 6
  • 5
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This EIF4B antibody is un-conjugated

Application

  • 83
  • 41
  • 36
  • 18
  • 17
  • 16
  • 13
  • 13
  • 6
  • 4
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
  • Binding Specificity

    • 20
    • 14
    • 8
    • 8
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 388-437, pSer422

    Specificity

    eIF4B (Phospho-Ser422) Antibody detects endogenous levels of eIF4B only when phosphorylated at Ser422.

    PhosphorylationH:S422 M:S422

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

    Comment

    Unigene-Number: Hs.207538 (NCBI Gene Symbol: EIF4B)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

    Alternative Name

    eIF4B

    Background

    Synonyms: Eukaryotic translation initiation factor 4B, IF4B, eIF-4B

    NCBI Gene Symbol: EIF4B

    Molecular Weight

    69 kDa

    Gene ID

    3716

    OMIM

    147795

    UniProt

    P23588
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