EIF4B antibody (pSer422)

Catalog No. ABIN1531431

The Rabbit Polyclonal anti-EIF4B antibody (ABIN1531431) specifically detects EIF4B in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for EIF4B antibody (pSer422) (ABIN1531431)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-EIF4B Antibody

Binding Specificity: AA 388-437, pSer422

Specificity:

eIF4B (Phospho-Ser422) Antibody detects endogenous levels of eIF4B only when phosphorylated at Ser422.

PhosphorylationH:S422 M:S422

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

Comment:

Unigene-Number: Hs.207538 (NCBI Gene Symbol: EIF4B)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: eIF4B

Background:

Synonyms: Eukaryotic translation initiation factor 4B, IF4B, eIF-4B

NCBI Gene Symbol: EIF4B

Molecular Weight: 69 kDa

Gene ID: 3716

OMIM: 147795

UniProt: P23588