YAP1 antibody (pSer127)

Catalog No. ABIN1531475

The Rabbit Polyclonal anti-YAP1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect YAP1 in samples from Human, Mouse and Rat.

Quick Overview for YAP1 antibody (pSer127) (ABIN1531475)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-YAP1 Antibody

Binding Specificity: AA 93-142, pSer127

Specificity:

YAP (Phospho-Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at Ser127.

PhosphorylationH:S127 M:S112 R:S112

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

Comment:

Unigene-Number: Hs.503692 (NCBI Gene Symbol: YAP1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP

Background:

Synonyms: 65 kDa Yes-associated protein, YAP1, YAP65

NCBI Gene Symbol: YAP1

Molecular Weight: 48 kDa

Gene ID: 10413

OMIM: 606608

UniProt: P46937

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha