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Histone H3.3 antibody (pSer31)

This Rabbit Polyclonal antibody specifically detects Histone H3.3 in ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.
Catalog No. ABIN1531501

Quick Overview for Histone H3.3 antibody (pSer31) (ABIN1531501)

Target

See all Histone H3.3 (H3F3A) Antibodies
Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity

  • 76
  • 16
  • 14
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Mouse, Rat

Host

  • 72
  • 9
Rabbit

Clonality

  • 58
  • 23
Polyclonal

Conjugate

  • 56
  • 6
  • 6
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
This Histone H3.3 antibody is un-conjugated

Application

  • 47
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  • 18
  • 14
  • 10
  • 8
  • 6
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunofluorescence (IF)
  • Binding Specificity

    • 11
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 16-65, pSer31

    Specificity

    Histone H3.3 (Phospho-Ser31) Antibody detects endogenous levels of Histone H3.3 only when phosphorylated at Ser31.

    PhosphorylationH:S31 M:S31 R:S31

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human Histone H3.3 around the phosphorylation site of Ser31.

    Isotype

    IgG
  • Application Notes

    IF: 1:100~1:500 ELISA: 1:20000

    Comment

    Unigene-Number: Hs.180877, Hs.533624, Hs.546259, Hs.699316, Hs.709407 (NCBI Gene Symbol: H3F3B)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

    Alternative Name

    Histone H3.3

    Background

    Synonyms: CG5825, H3.3Q, H3.A, H3.B, H33, H3F3A, H3F3B, HIS3.3A, HIS3.3B, ORCG8989, HISH3-3Q, Histone H3.3

    NCBI Gene Symbol: H3F3B

    Molecular Weight

    15 kDa

    Gene ID

    3020, 3021

    OMIM

    601058

    UniProt

    P84243
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