Histone H3.3 antibody (pSer31)

Catalog No. ABIN1531501

This Rabbit Polyclonal antibody specifically detects Histone H3.3 in ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Histone H3.3 antibody (pSer31) (ABIN1531501)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-Histone H3.3 Antibody

Binding Specificity: AA 16-65, pSer31

Specificity:

Histone H3.3 (Phospho-Ser31) Antibody detects endogenous levels of Histone H3.3 only when phosphorylated at Ser31.

PhosphorylationH:S31 M:S31 R:S31

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3.3 around the phosphorylation site of Ser31.

Isotype: IgG

Application Details

Application Notes: IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.180877, Hs.533624, Hs.546259, Hs.699316, Hs.709407 (NCBI Gene Symbol: H3F3B)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histone H3.3

Background:

Synonyms: CG5825, H3.3Q, H3.A, H3.B, H33, H3F3A, H3F3B, HIS3.3A, HIS3.3B, ORCG8989, HISH3-3Q, Histone H3.3

NCBI Gene Symbol: H3F3B

Molecular Weight: 15 kDa

Gene ID: 3020, 3021

OMIM: 601058

UniProt: P84243