PKC alpha antibody (pTyr657)

Catalog No. ABIN1531505

The Rabbit Polyclonal anti-PKC alpha antibody has been validated for WB and ELISA. It is suitable to detect PKC alpha in samples from Human, Mouse and Rat.

Quick Overview for PKC alpha antibody (pTyr657) (ABIN1531505)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PKC alpha Antibody

Binding Specificity: AA 623-672, pTyr657

Specificity:

PKC alpha (Phospho-Tyr657) Antibody detects endogenous levels of PKC alpha only when phosphorylated at Tyr657.

PhosphorylationH:Y657 M:Y657 R:Y657

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Tyr657.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:1000

Comment:

Unigene-Number: Hs.531704, Hs.708867 (NCBI Gene Symbol: PRKCA)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PKC alpha

Background:

Synonyms: kinase PKC-alpha, KPCA, PKC III, PKC-A, PKC-alpha, PKC-III, PRKACA, PRKCA, Protein kinase C, alpha type

NCBI Gene Symbol: PRKCA

Molecular Weight: 76 kDa

Gene ID: 5578

OMIM: 176960

UniProt: P17252

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling