CD28 antibody (pTyr218)

Catalog No. ABIN1531543

This Rabbit Polyclonal antibody specifically detects CD28 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for CD28 antibody (pTyr218) (ABIN1531543)

Target: CD28

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD28 Antibody

Binding Specificity: AA 171-220, pTyr218

Specificity:

CD28 (Phospho-Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.

PhosphorylationH:Y218 M:Y216

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:40000

Comment:

Unigene-Number: Hs.591629 (NCBI Gene Symbol: CD28)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background:

Synonyms: SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen

NCBI Gene Symbol: CD28

Molecular Weight: 25 kDa

Gene ID: 940

OMIM: 186760

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response