CD5 antibody (pTyr453)

Catalog No. ABIN1531547

The Rabbit Polyclonal anti-CD5 antibody has been validated for WB, IF and ELISA. It is suitable to detect CD5 in samples from Human, Mouse and Rat.

Quick Overview for CD5 antibody (pTyr453) (ABIN1531547)

Target: CD5

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Product Details anti-CD5 Antibody

Binding Specificity: AA 421-470, pTyr453

Specificity:

CD5 (Phospho-Tyr453) Antibody detects endogenous levels of CD5 only when phosphorylated at Tyr453.

PhosphorylationH:Y453 M:Y452 R:Y449

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CD5 around the phosphorylation site of Tyr453.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.58685 (NCBI Gene Symbol: CD5)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background:

Synonyms: LEU1, Lymphocyte antigen CD5, Lymphocyte glycoprotein Ly-1, Lymphocyte glycoprotein T1/Leu-1, Lyt-1, T-cell surface glycoprotein CD5 precursor

NCBI Gene Symbol: CD5

Molecular Weight: 54 kDa

Gene ID: 921

OMIM: 153340

UniProt: P06127