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EPHA2/3 antibody (pTyr588)

The Rabbit Polyclonal anti-EPHA2/3 antibody has been validated for WB, ELISA and IF. It is suitable to detect EPHA2/3 in samples from Human and Rat.
Catalog No. ABIN1531586

Quick Overview for EPHA2/3 antibody (pTyr588) (ABIN1531586)

Target

EPHA2/3 (Eph Receptor A2+A3 (EPHA2/3))

Reactivity

  • 17
  • 15
  • 2
Human, Rat

Host

  • 17
Rabbit

Clonality

  • 17
Polyclonal

Conjugate

  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This EPHA2/3 antibody is un-conjugated

Application

  • 17
  • 13
  • 13
  • 5
  • 3
  • 3
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF)
  • Binding Specificity

    • 17
    • 16
    • 1
    AA 556-605, pTyr588

    Specificity

    EPHA2/3 (Phospho-Tyr588/596) Antibody detects endogenous levels of EPHA2/3 only when phosphorylated at Tyr588/596.

    PhosphorylationH:Y588/Y596M:Y589/Y596 R:-/Y597

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human EPHA2/3 around the phosphorylation site of Tyr588/596.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

    Comment

    Unigene-Number: Hs.171596, Hs.123642, Hs.371218 (NCBI Gene Symbol: EPHA2)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    EPHA2/3 (Eph Receptor A2+A3 (EPHA2/3))

    Background

    Synonyms: ECK, Ephrin type-A receptor 2 precursor, Epithelial cell kinase, MPK-5, SEK-2, SEK2, Tyrosine-protein kinase receptor ECK, kinase EphA2

    NCBI Gene Symbol: EPHA2

    Molecular Weight

    108 kDa

    Gene ID

    1969, 2042, 2043

    OMIM

    176946

    UniProt

    P29317, P29320, P54764
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