MAPK15 antibody (pThr175)
Quick Overview for MAPK15 antibody (pThr175) (ABIN1531592)
Target
See all MAPK15 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 141-190, pThr175
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Specificity
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ERK8 (Phospho-Thr175+Tyr177) Antibody detects endogenous levels of ERK8 only when phosphorylated at Thr175 and Tyr177.
PhosphorylationH:T175+Y177 M:T176+Y178
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Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Purity
- > 95 %
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Immunogen
- The antiserum was produced against synthesized peptide derived from human ERK8 around the phosphorylation site of Thr175 and Tyr177.
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Isotype
- IgG
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Application Notes
- WB: 1:500~1:1000 ELISA: 1:5000
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Comment
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Unigene-Number: Hs.493169 (NCBI Gene Symbol: MAPK15)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Stable at -20°C for at least 1 year.
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Expiry Date
- 12 months
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- MAPK15 (Mitogen-Activated Protein Kinase 15 (MAPK15))
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Alternative Name
- ERK8
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Background
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Synonyms: Extracellular signal-regulated kinase 8
NCBI Gene Symbol: MAPK15
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Molecular Weight
- 59 kDa
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Gene ID
- 225689
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UniProt
- Q8TD08
Target
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