ASK1 antibody (pSer83)

Catalog No. ABIN1531772

The Rabbit Polyclonal anti-ASK1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect ASK1 in samples from Human.

Quick Overview for ASK1 antibody (pSer83) (ABIN1531772)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ASK1 Antibody

Binding Specificity: AA 49-98, pSer83

Specificity:

ASK1 (Phospho-Ser83) Antibody detects endogenous levels of ASK1 only when phosphorylated at Ser83.

PhosphorylationH:S83

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser83.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.186486 (NCBI Gene Symbol: MAP3K5)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: ASK1

Background:

Synonyms: Apoptosis signal-regulating kinase 1, ASK-1, kinase ASK1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Mitogen-activated protein kinase kinase kinase 5

NCBI Gene Symbol: MAP3K5

Molecular Weight: 154 kDa

Gene ID: 4217

OMIM: 602448

UniProt: Q99683

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response