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EIF4E antibody (pSer209)

This Rabbit Polyclonal antibody specifically detects EIF4E in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.
Catalog No. ABIN1531825

Quick Overview for EIF4E antibody (pSer209) (ABIN1531825)

Target

See all EIF4E Antibodies
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity

  • 124
  • 62
  • 50
  • 19
  • 5
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
Human, Rat, Mouse

Host

  • 108
  • 15
  • 2
Rabbit

Clonality

  • 98
  • 27
Polyclonal

Conjugate

  • 59
  • 9
  • 8
  • 6
  • 5
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This EIF4E antibody is un-conjugated

Application

  • 98
  • 51
  • 43
  • 28
  • 27
  • 26
  • 16
  • 16
  • 16
  • 14
  • 7
  • 6
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • Binding Specificity

    • 39
    • 15
    • 8
    • 7
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 168-217, pSer209

    Specificity

    eIF4E (Phospho-Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at Ser209.

    PhosphorylationH:S209 M:S209 R:S209

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human eIF4E around the phosphorylation site of Ser209.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

    Comment

    Unigene-Number: Hs.249718 (NCBI Gene Symbol: EIF4E)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    Alternative Name

    eIF4E

    Background

    Synonyms: Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, mRNA cap-binding protein, eIF-4F 25 kDa subunit, EIF4E, EIF4EL1, EIF4F

    NCBI Gene Symbol: EIF4E

    Molecular Weight

    25 kDa

    Gene ID

    1977

    OMIM

    133440

    UniProt

    P06730

    Pathways

    BCR Signaling
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