EIF4E antibody (pSer209)
Quick Overview for EIF4E antibody (pSer209) (ABIN1531825)
Target
See all EIF4E AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 168-217, pSer209
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Specificity
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eIF4E (Phospho-Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at Ser209.
PhosphorylationH:S209 M:S209 R:S209
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Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Purity
- > 95 %
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Immunogen
- The antiserum was produced against synthesized peptide derived from human eIF4E around the phosphorylation site of Ser209.
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Isotype
- IgG
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Application Notes
- WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
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Comment
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Unigene-Number: Hs.249718 (NCBI Gene Symbol: EIF4E)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Stable at -20°C for at least 1 year.
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Expiry Date
- 12 months
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- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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Alternative Name
- eIF4E
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Background
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Synonyms: Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, mRNA cap-binding protein, eIF-4F 25 kDa subunit, EIF4E, EIF4EL1, EIF4F
NCBI Gene Symbol: EIF4E
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Molecular Weight
- 25 kDa
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Gene ID
- 1977
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OMIM
- 133440
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UniProt
- P06730
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Pathways
- BCR Signaling
Target
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