EIF4E antibody (pSer209)

Catalog No. ABIN1531825

This Rabbit Polyclonal antibody specifically detects EIF4E in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for EIF4E antibody (pSer209) (ABIN1531825)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-EIF4E Antibody

Binding Specificity: AA 168-217, pSer209

Specificity:

eIF4E (Phospho-Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at Ser209.

PhosphorylationH:S209 M:S209 R:S209

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human eIF4E around the phosphorylation site of Ser209.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.249718 (NCBI Gene Symbol: EIF4E)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: eIF4E

Background:

Synonyms: Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, mRNA cap-binding protein, eIF-4F 25 kDa subunit, EIF4E, EIF4EL1, EIF4F

NCBI Gene Symbol: EIF4E

Molecular Weight: 25 kDa

Gene ID: 1977

OMIM: 133440

UniProt: P06730

Pathways: BCR Signaling