Ezrin antibody (pTyr478)
Quick Overview for Ezrin antibody (pTyr478) (ABIN1532010)
Target
See all Ezrin (EZR) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 446-495, pTyr478
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Specificity
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Ezrin (Phospho-Tyr478) Antibody detects endogenous levels of Ezrin only when phosphorylated at Tyr478.
PhosphorylationH:Y478 M:Y478 R:Y478
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Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Purity
- > 95 %
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Immunogen
- The antiserum was produced against synthesized peptide derived from human Ezrin around the phosphorylation site of Tyr478.
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Isotype
- IgG
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Application Notes
- WB: 1:500~1:1000 ELISA: 1:1000
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Comment
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Unigene-Number: Hs.487027 (NCBI Gene Symbol: EZR)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Stable at -20°C for at least 1 year.
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Expiry Date
- 12 months
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- Ezrin (EZR)
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Alternative Name
- Ezrin
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Background
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Synonyms: Cytovillin, EZRI, p81, VIL2, Villin 2
NCBI Gene Symbol: EZR
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Molecular Weight
- 69 kDa
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Gene ID
- 7430
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OMIM
- 123900
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UniProt
- P15311
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Pathways
- Maintenance of Protein Location
Target
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