AKT 1/2/3 antibody (pTyr315)
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- Target See all AKT 1/2/3 products
- AKT 1/2/3
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Binding Specificity
- AA 281-330, pTyr315
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This AKT 1/2/3 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), ELISA
- Specificity
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AKT1/2/3 (Phospho-Tyr315/316/312) Antibody detects endogenous levels of AKT1/2/3 only when phosphorylated at Tyr315/316/312.
PhosphorylationH:Y315/316/312 M:Y315/316/312 R:Y315/316/312
- Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
- Purity
- > 95 %
- Immunogen
- The antiserum was produced against synthesized peptide derived from human AKT1/2/3 around the phosphorylation site of Tyr315/316/312.
- Isotype
- IgG
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- Application Notes
- IHC: 1:50~1:100 ELISA: 1:20000
- Comment
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Unigene-Number: Hs.525622, Hs.631535, Hs.498292 (NCBI Gene Symbol: AKT1)
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Stable at -20°C for at least 1 year.
- Expiry Date
- 12 months
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- Target
- AKT 1/2/3
- Alternative Name
- AKT1/2/3 (AKT 1/2/3 Products)
- Background
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Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT/ RAC-PK-beta, Protein kinase Akt-2, Protein kinase B, beta, PKB beta/ RAC-PK-gamma, Protein kinase Akt-3, Protein kinase B, gamma, PKB gamma, STK-2
NCBI Gene Symbol: AKT1
- Molecular Weight
- 55 kDa
- Gene ID
- 207, 208, 10000
- OMIM
- 164730
- UniProt
- P31749, P31751, Q9Y243
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