NOS2 antibody (pTyr151)

Catalog No. ABIN1532100

This Rabbit Polyclonal antibody specifically detects NOS2 in IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NOS2 antibody (pTyr151) (ABIN1532100)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-NOS2 Antibody

Binding Specificity: AA 117-166, pTyr151

Specificity:

iNOS (Phospho-Tyr151) Antibody detects endogenous levels of iNOS only when phosphorylated at Tyr151.

PhosphorylationH:Y151 M:Y145 R:Y151

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human iNOS around the phosphorylation site of Tyr151.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.709191 (NCBI Gene Symbol: NOS2)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: iNOS

Background:

Synonyms: HEP-NOS, hepatocyte NOS, inducible NOS, nitric oxide synthase, inducible, NOS type II, NOS2, NOS2A

NCBI Gene Symbol: NOS2

Molecular Weight: 131 kDa

Gene ID: 4843

OMIM: 163730

UniProt: P35228

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process