Histone H2A antibody (pThr121)

Catalog No. ABIN1532108

This anti-Histone H2A antibody is a Rabbit Polyclonal antibody detecting Histone H2A in IHC and ELISA. Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for Histone H2A antibody (pThr121) (ABIN1532108)

Target: Histone H2A

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-Histone H2A Antibody

Binding Specificity: AA 81-130, pThr121

Specificity:

Histone H2A (Phospho-Thr121) Antibody detects endogenous levels of Histone H2A only when phosphorylated at Thr121.

PhosphorylationH:T121 M:T121 R:T121

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H2A around the phosphorylation site of Thr121.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:1000

Comment:

Unigene-Number: Hs.134999, Hs.233568, Hs.51011, Hs.534035, Hs.558421, Hs.121017, Hs.248174, Hs.679229, Hs.352225, Hs.530461, Hs.706714, Hs.26331, Hs.700190 (NCBI Gene Symbol: HIST1H2AB)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-Histone H2A antibody (ABIN1532108)

Baron, von Schubert, Cubizolles, Siemeister, Hitchcock, Mengel, Schröder, Fernández-Montalván, von Nussbaum, Mumberg, Nigg: "Probing the catalytic functions of Bub1 kinase using the small molecule inhibitors BAY-320 and BAY-524." in: eLife, Vol. 5, (2017) (PubMed).

Target Details for Histone H2A

Target: Histone H2A

Background:

Synonyms: H2A.1b, H2A/c, H2A/d, H2A/i, H2A/n, H2A/p, H2A1, H2AC, H2AFC, H2AFD ANDHIST1H2AL, H2AFI, H2AFN ANDHIST1H2AG, H2AFP, HIST1H2AI, HIST1H2AJ, HIST1H2AM, Histone H2A.c/d/i/n/p

NCBI Gene Symbol: HIST1H2AB

Molecular Weight: 14 kDa

Gene ID: 8329, 8332, 8336

OMIM: 602787

UniProt: P0C0S8, P04908, P20671, Q96KK5, Q6FI13, Q7L7L0