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Vinculin antibody (AA 786-835)

The Rabbit Polyclonal anti-Vinculin antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect Vinculin in samples from Human, Mouse and Rat.
Catalog No. ABIN1532662

Quick Overview for Vinculin antibody (AA 786-835) (ABIN1532662)

Target

See all Vinculin (VCL) Antibodies
Vinculin (VCL)

Reactivity

  • 148
  • 92
  • 70
  • 28
  • 18
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Human, Mouse, Rat

Host

  • 99
  • 53
  • 1
Rabbit

Clonality

  • 86
  • 67
Polyclonal

Conjugate

  • 97
  • 9
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
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  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Vinculin antibody is un-conjugated

Application

  • 115
  • 67
  • 32
  • 29
  • 27
  • 22
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  • 21
  • 20
  • 14
  • 13
  • 9
  • 4
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • Binding Specificity

    • 26
    • 16
    • 9
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 786-835

    Specificity

    Vinculin Antibody detects endogenous levels of total Vinculin protein.

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human Vinculin.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

    Comment

    Unigene-Number: Hs.643896 (NCBI Gene Symbol: VINC)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    Vinculin (VCL)

    Alternative Name

    Vinculin

    Background

    Synonyms: Metavinculin, VCL, VINC

    NCBI Gene Symbol: VINC

    Molecular Weight

    123 kDa

    Gene ID

    7414

    OMIM

    193065

    UniProt

    P18206

    Pathways

    Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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