Histone H1 antibody (acLys25)
Quick Overview for Histone H1 antibody (acLys25) (ABIN1535513)
Target
See all Histone H1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 131-180, acLys25
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Specificity
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Histone H1 (Acetyl-Lys25) Antibody detects endogenous levels of total Histone H1 protein only when acetylated at Lys25.
AcetylationH:K25
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Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
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Purity
- > 95 %
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Immunogen
- The antiserum was produced against synthesized peptide derived from human Histone H1 around the acetylated site of Lys25.
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Isotype
- IgG
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Application Notes
- WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
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Comment
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Unigene-Number: Hs.97358 (NCBI Gene Symbol: H1FOO)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Stable at -20°C for at least 1 year.
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Expiry Date
- 12 months
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- Histone H1
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Background
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Synonyms: H1, H1 histone family member 5, H1.5, H15, H1F5, HIST1H1B, histone 1, histone H1.5, histone H1a
NCBI Gene Symbol: H1FOO
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Molecular Weight
- 21 kDa
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Gene ID
- 132243
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UniProt
- Q8IZA3
Target
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