Histone H4 antibody (acLys12)
Quick Overview for Histone H4 antibody (acLys12) (ABIN1535525)
Target
See all Histone H4 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 10-59, acLys12
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Specificity
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Histone H4 (Acetyl-Lys12) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at Lys12.
AcetylationH:K12 M:K12 R:K12
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Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
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Purity
- > 95 %
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Immunogen
- The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12.
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Isotype
- IgG
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Application Notes
- WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
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Comment
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Unigene-Number: Hs.143080, Hs.247816, Hs.248172, Hs.248178, Hs.248179, Hs.278483, Hs.46423, Hs.528055, Hs.533295, Hs.55468, Hs.591790, Hs.655235, Hs.662174, Hs.706635, Hs.718935 (NCBI Gene Symbol: HIST1H4A)554313 (NCBI Gene Symbol: HIST1H4B)8294 (NCBI Gene Symbol: HIST1H4C)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Stable at -20°C for at least 1 year.
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Expiry Date
- 12 months
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- Histone H4
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Background
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Synonyms: H4F2, HIST, HIST1H4A, Histone H4
NCBI Gene Symbol: HIST1H4A/HIST1H4B/HIST1H4C/HIST1H4D/HIST1H4E/HIST1H4F/HIST1H4H/HIST1H4I/HIST1H4J/HIST1H4K/HIST1H4L/HIST2H4A/HIST2H4B/HIST4H4
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Molecular Weight
- 11 kDa
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Gene ID
- 121504, 554313, 8294
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OMIM
- 142750
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UniProt
- P62805
Target
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