Topoisomerase II alpha antibody

Catalog No. ABIN1539957

This anti-Topoisomerase II alpha antibody is a Mouse Monoclonal antibody detecting Topoisomerase II alpha in WB. Suitable for Human.

Quick Overview for Topoisomerase II alpha antibody (ABIN1539957)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 548CT6-1-1

Product Details anti-Topoisomerase II alpha Antibody

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Immunogen: Purified His-tagged TOP2A protein(Fragment) was used to produced this monoclonal antibody.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500~8000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: TOP2A Antibody can be refrigerated at 2-8 °C for up to 6 months. For long term storage, keep at -20 °C.

Expiry Date: 6 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Molecular Weight: 174385

Gene ID: 7153

NCBI Accession: NP_001058

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases