RYBP antibody

Catalog No. ABIN155069

Product Details anti-RYBP Antibody

Target: RYBP (RING1 and YY1 Binding Protein (RYBP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RYBP antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects DEDAF. A 32 kDa band should be detected.

Purification: Affinity purified

Immunogen: Synthetic peptide surrounding amino acid 218 of human DEDAF.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. A549 or HepG2 cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for RYBP

Target: RYBP (RING1 and YY1 Binding Protein (RYBP))

Alternative Name: RYBP (RYBP Products)

Synonyms: AAP1 antibody, DEDAF antibody, YEAF1 antibody, 2410018J24Rik antibody, rybp antibody, aap1 antibody, dedaf antibody, yeaf1 antibody, cb337 antibody, wu:fi34f03 antibody, Dedaf antibody, zgc:153842 antibody, RING1 and YY1 binding protein antibody, RING1 and YY1 binding protein L homeolog antibody, RING1 and YY1 binding protein a antibody, RING1 and YY1 binding protein b antibody, RYBP antibody, Rybp antibody, rybp antibody, rybp.L antibody, rybpa antibody, rybpb antibody

Background: Cell death signal are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. A novel protein that interacts with DED of caspase-8 and -10, and FADD was recently identified and designated DEDAF (for DED associated factor). DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/caspase-8 complexes at the cytoplasm. DEDAF also interacts with DED-containing DNA biding protein (DEDD) in the nucleus, indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.Synonyms: AAP-1, APAP-1, Apoptin-associating protein 1, DED-associated factor, DEDAF, Death effector domain-associated factor, RING1 and YY1-binding protein, YEAF1, YY1 and E4TF1-associated factor 1

Gene ID: 23429

NCBI Accession: NP_036366

UniProt: Q8N488