B Tag antibody

Catalog No. ABIN1573911

This Rabbit Polyclonal antibody specifically detects B Tag in ELISA and WB. It has been mentioned in 1 publication

Quick Overview for B Tag antibody (ABIN1573911)

Target: B Tag

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Product Details

Sequence: QYPALT

Specificity: Rabbit Anti-B-tag Polyclonal Antibody recognizes either N-terminal or C-terminal B tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogen: B epitope tag peptide QYPALT conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using the ONE-HOUR WesternTM Kit: For quick results, the ONE-HOUR Western^TM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for an 8 cm X 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References

Lee, Chen, Lin, Wang: "Presence of bluetongue virus in the marginal zone of the spleen in acute infected sheep." in: Veterinary microbiology, Vol. 152, Issue 1-2, pp. 96-100, (2011) (PubMed).

Target Details

Target: B Tag

Alternative Name: B-Tag

Target Type: Tag

Background: Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The B-tag is an epitope tag composed of a six-residue peptide, QYPALT, corresponding to a highly conserved region of VP7 core protein of the blue tongue virus. The Rabbit Anti-B-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic B-tag peptide conjugated to KLH.Rabbit Anti-B-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml.